Synthesis of Silver Nanoparticles using Klebsiella pneumonia and its Bio-Medical Applications
Author Name : Amar Ratan, Ekta Gupta, R. Ragunathan
The antimicrobial effects of sliver ions have been known for long but their activity against microorganisms has not yet been conclusively studied. In the present study, biological synthesis of silver nanoparticles using Klebsiella pneumonia has been investigated. The bacterial strain was grown the appropriate production media. The cell free filtrate obtained on filtration was bought in contact with different concentrations of AgNO3. . The change in the pH with respect to the different concentrations was studied .The nanoparticles were further characterized by SEM studies. The antimicrobial activity of silver nanoparticles was investigated against E. coli, Bacillus and ESBL .Also the antimicrobial activity of encapsulated beads of silver nanoparticles and 2mM silver nanoparticles was compared. The anti-microbial fabrics activity was studied by coating the silver nanoparticles on gauge cotton fabric and then compared with standard disc Oxacillin. UV-VIS studies showed an increased in absorbance at 363 nm. The antimicrobial tests against E.coli showed increased inhibition at 1mM while for Bacillus, inhibition was more prominent at 2mM concentration and for ESBL-Pseudomonas inhibitory concentration was 2mM .The zone of inhibition for the encapsulated beads was less than that of the 2mM silver nanoparticles.