A Comprehensive Review on UV-Visible Spectros...

A Comprehensive Review on UV-Visible Spectroscopy and Its application

Author Name : Suman Ghosh, Sumit Nandi

DOI: https://doi.org/10.56025/IJARESM.2023.1201241025

ABSTRACT The review highlights with an exploration of the fundamentals, explaining how molecules interact with ultraviolet or visible light and the ensuing electronic transitions that result in absorbance spectra. We go into great detail about the Beer-Lambert Law, which governs the quantitative aspects of UV-visible spectroscopy and emphasizes its significance in concentration determinations. A mainstay of analytical methods, UV-visible spectroscopy provides a plethora of information about chemical structures, electronic transitions, and material concentrations for use in a wide range of scientific fields. This thorough examination illuminates the many uses of UV-visible spectroscopy by delving into its underlying theories and methods. After that, the review provides a thorough overview of the various fields in which UV-visible spectroscopy is used. The method is essential for quantitative analysis in chemistry because it makes concentration determination and reaction kinetics research possible. Protein and nucleic acid analysis is used in biochemical and biomedical applications, and UV spectroscopy is used in the pharmaceutical business for drug formulation and quality assurance. Further investigated are the fields of chemical kinetics, forensic analysis, and clinical diagnostics where UV-visible spectroscopy is essential. The review ends by highlighting the UV-visible spectroscopy's on-going importance in research, education, and industrial applications, demonstrating its versatility and on-going relevance in promoting scientific knowledge and technological advancement. For scientists, researchers, educators, and industry professionals looking for a deeper understanding of UV-visible spectroscopy, its principles, instrumentation, and the many applications that have led to its reputation as a cornerstone analytical technique, this thorough review is an invaluable resource.