A Review on LCMS/ MS Instrumentation, HPLC- G...

A Review on LCMS/ MS Instrumentation, HPLC- GC and guidelines for Pharmaceutical Industry

Author Name : Pratiksha P. Yawalkar, K.B. Gabhane

ABSTRACT

A range of analytical techniques are available for bioanalysis, including HPLC apparatus for analyte detection and LCMS/MS. The current study describes a method development strategy for 4-dimethylaminopyridine trace analysis utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) and design of experiments (DoE) (DMAP) Method validation is a methodology for determining whether a method will effectively meet the FDA's minimum requirements. To quantify drugs in biological samples such as blood, plasma, serum, or urine, extremely sensitive and selective technologies are necessary in today's drug environment. For the bioanalysis of tiny compounds, chromatographic methods (high-performance liquid chromatography [HPLC] or gas chromatography [GC]) have been widely employed, with liquid chromatography coupled to mass spectrometry (LC/MS/MS) being the most popular. After creating a method with the needed attributes, it is validated for factors such as accuracy, precision, and so on.

Key Words: LCMS/MS, HPLC and GC instrumentation, Guidelines.