Development and Validation of Rp-Hplc Method ...

Development and Validation of Rp-Hplc Method for the Estimation of Clenbuterol Hydrochloride in Bulk And Tablet

Author Name : Akshata Khadke, Smita Kumbhar, Poonam Katmore

ABSTRACT

The purpose of this analysis study is to develop a novel, simple, accurate, precise, specific and economical method for RP-HPLC to determination of the connected substances in tablet and bulk form of clenbuterol hydrochloride. Agilent zorbax bonus-RP C18 (250× 4.6 mm, 5µ) column using for chromatographic separation. Mobile part is 0.1% TFA water : acetonitrile (60:40v/v) at a flow rate 1 ml/min. The linearity range of concentration 25-75µg/ml. 3.87 min is retention time and ultraviolet detector at 245nm with 10µL injection volume. With 8 min run time. Clenbuterol hydrochloride valid as per guidelines of ICH with respect of specificity, accuracy, linearity and precision.

Keywords: Clenbuterol hydrochloride, RP-HPLC, Method development, 245 nm