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HPTLC-Based Quantitative Analysis Of Abrocitinib In Oral Dosage Form: Method Development And Validation Approach
Author Name : Shifa Khan, Shreya Thakker, Prajyot Mali, Vijay Kumar Munipalli, Bhushan Sonawane
ABSTRACT: A novel accurate, sensitive, reproducible and reliable High Performance Thin Layer Chromatography method was developed and validated for the determination and estimation of Abrocitinib i.e. (Active Pharmaceutical Ingredient) in tablet dosage form. Chromatographic separation of Abrocitinib was obtained on Silica gel 60 F254 HPTLC plates as the stationary phase using a standardized mobile phase of Ethyl acetate: Chloroform: Methanol: Ammonia (4:3:1.5:1.5 v/v/v/v). Two immiscible layers are formed after addition of aforementioned ratios. From this combined layer, the organic layer was collected cautiously.