Perturbations of Zinc And Vitamin-C Effect on...

Perturbations of Zinc And Vitamin-C Effect on Cadmium Induced Oxidative Stress in Male Albino Rat

Author Name : Vasuru Kesavulu, Asupatri Usha Rani*

ABSTRACT

Cadmium (Cd) is a one of the toxic heavy metals that shows toxicological effects on different organs and tissues of different animal models and humans. In this work, we explored the effects of Zinc and Vitamin C in reducing cadmium induced oxidative stress in male albino rat, particularly in the organs of the liver, kidney and testis. To achieve this objective, Wister strain male albino rats were treated with cadmium chloride (CdCl₂) at a dose of 1/10^th LD50/48 h i.e. that is 22.5 mg/kg body weight for 7, 15 and 30 days (d). Later 15d Cd treated rats were divided into three groups. The 1^st groups of rats were subjected to Zinc (12mg/kg), 2^nd group to Vitamin-C (200 mg/kg) and the 3^rd group to combination of Zinc and Vitamin-C supplementation for 7, 15 and 30d time periods. After the specific time intervals, rats were sacrificed and oxidative stress enzyme like catalase (CAT), superoxide dismutase (SOD), xanthine oxidase (XOD), glutathione peroxidase (GPX), and glutathione–S–transferase (GST) were estimated in the liver, kidney and testis. Simultaneously lipid peroxidation lipid peroxidation (LPO) levels were also measured. A significant elevation in LPO and XOD levels with decreased activity levels of CAT, SOD, GST and GPX were observed during Cd intoxication. The results of this research demonstrated a high level of the total antioxidant capacity of the intact cells of the Zn and Vitamin C supplementation. Therefore, we conclude that a combination of Zn and Vitamin C supplementation is effective in detoxifying the Cd body burden from the test tissue.

Keywords: Cadmium, Kidney, Liver, Oxidative stress, Rat, Testis.