Phytochemical Analysis and Antioxidant Activi...

Phytochemical Analysis and Antioxidant Activity of Euphorbia hirta L. Leaves Extract

Author Name : Utsav Gangwar, Garima Gupta, Anil Kumar, Amit Kumar

ABSTRACT

In this current research, a detailed protocol has been programmed. The leaves of Euphorbia hirta were collected from nearby areas of Delhi in December. An herbarium was prepared and authenticated from NISCAIR, Delhi. Dried leaves were extracted using solvent ethanol by the soxhlet apparatus. The extract was dried using a rotary evaporator. The obtained extract was subjected to preliminary phytochemical tests. Qualitative phytochemical tests confirmed the presence of alkaloids, saponins, flavonoids, steroids, Terpines, Phenolics and Tannins. Quantitative phytochemical investigation revealed the 0.53±0.12 Phenols, 10.2±0.15% Alkaloids, 2.3±0.11% Tannins, 0.51±0.20% Saponins, 08.719±0.23% Flavonoids. HPTLC fingerprinting study showed that ethanolic extract contain quercetin. The presence of phenols and flavonoids in the plant plays a major role in controlling antioxidants. It contains quercetin which is responsible for higher antioxidant activity.

Keywords: Euphorbia hirta, HPTLC, quercetin, antioxidant activity.