Review: Basics, Role of RP-HPLCIN Analytical ...

Review: Basics, Role of RP-HPLCIN Analytical and Bio Analytical Method Development and Recent Trends in RP-HPLC

Author Name : Vaishnavi Malve, Isha Joshi, S. G. Jawarkar, A.W. Baitule

ABSTRACT

Although chromatography is primarily a separation technique, it is most commonly used in chemical analysis. High-performance liquid chromatography (HPLC) is a very adaptable technique that separates analytes by passing them through a column packed with micrometre-sized particles. In today's HPLC, reversed-phase chromatography is the most often utilised separation technology. The reversed phase method's simplicity, versatility, and scope are reasons for this, as it can handle compounds with a wide range of polarity and molecular mass. In the field of biological separation and purification, reversed phase chromatography has analytical and preparative applications. Reversed phase chromatography can separate molecules with some degree of hydrophobicity, such as proteins, peptides, and nucleic acids, with great recovery and resolution. This paper discusses the importance of RP-HPLC in the development of analytical methods and their methodologies, as well as a quick overview of essential chromatographic parameters that must be optimised for effective method development.

Key words: HPLC, RP-HPLC, critical parameters, Green Chromatography