“RP-HPLC method development and validation ...

“RP-HPLC method development and validation for simultaneous estimation of Choline Salicylate and Lignocaine hydrochloride in oral Gel”

Author Name : Patel Visha Jitendrakumar, Dr. Alisha Patel

ABSTRACT

A simple, rapid, precise and accurate high performance liquid chromatography method was developed for simultaneous estimation of Choline Salicylate and Lignocaine Hydrochloride in gel. The separation was obtained using a mobile phase consisting of Acetonitrile: Methanol: Water (pH 3 adjusted with 1% OPA) (35:35:30 %v/v/v)using Shim-pack solar C18 (250 × 4.6 mm, 5 µm) column. The flow rate 1.0 ml /min and UV detection at 225 nm was employed. The retention time for Choline Salicylate and Lignocaine Hydrochloride was 4.365 min and 2.403 min respectively. Linearity for Choline Salicylate and Lignocaine Hydrochloride was found to be in the range of 64-174 µg/ml and 15-40 µg/ml respectively.Assay for Choline Salicylate and Lignocaine Hydrochloride was found to be 99.91% and 99.75% respectively. The method was validated as per the ICH guidelines and the results were within the acceptance criteria for precision, linearity, specificity, and robustness.

Key words: Choline Salicylate, Lignocaine Hydrochloride, RP-HPLC, Simultaneous estimation