Standardization of Rapid Blood Isolation Prot...

Standardization of Rapid Blood Isolation Protocol for PCR Analysis

Author Name : Dharmendra Mishra, Dr. Abhijit Sahasrabudhe

ABSTRACT

Pure DNA isolation is the first and crucial step for many of the available downstream applications used in the field of molecular biology Whole blood samples are one of the main sources used to obtain DNA, and there are many different protocols available to perform nucleic acid extraction on such samples. These methods vary from very basic manual protocols to more sophisticated methods included in automated DNA extraction protocols. Based on the wide range of available options, it would be ideal to determine the ones that perform best in terms of cost-effectiveness and time efficiency. We have standardized blood DNA isolation with few modifications in SDS based method. The key features are during isolation we have avoided the use of hazardous chemicals such as phenol, chloroform: isoamylalcohol etc. Without the use of RNase we were able to isolate pure and sufficient amount of DNA which proved to be ideal for PCR analysis.

Keywords: Whole blood sample, genomic DNA isolation, without RNase, conventional PCR