The Concept and implementation of doubled hap...

The Concept and implementation of doubled haploid in Crops- A critical Review

Author Name : Sumati Devi Potsangbam, Manoj Kumar Pandey

ABSTRACT

The production of double haploid has become very important tools for commercial production in large scale. An efficient double haploid production can reduce time and cost of cultivation. With the development of technology in molecular level, we can develop a proper homozygous from haploid cells. By undertaking this, it will save six long years which would take if conventional breeding procedure is followed; it will take up only up to a year for production of double haploid. Doubled haploid is the production of genotypes using only the haploids cell obtains from egg cells or anthers or other cells from gametophyte and inducing double chromosome to make up the original chromosome number. So, there are usually two processes to get double haploids, namely: getting haploid cells and inducing chromosome doubling. Performing doubled haploids helps us to efficiently study in the molecular level, save time from performing the shortest way than using the traditional longest way, getting the required desired genotypes as we selected only the haploid and doubling them. All the concepts of performing and implementations of doubled haploid are going to study and observe in this review paper by performing anther culture, through androgenesis in microspore culture, using colchicine. DH techniques can be performed both inin vivoorin vitro conditions. It can be compared to single seed decent method and pure line selection.

Keywords: Doubled haploids, Anther culture, Colchicine.