A Comparative Study on Analytical Methods by ...

A Comparative Study on Analytical Methods by Infrared Spectroscopy (IR) and High-Performance Liquid Chromatography (HPLC) By Estimation of Azithromycin

Author Name : Dr. R. Manivannan, Mr. N. Elavarasan, K. Sindhuja, V. Sriban, A. Tamilarasu, R. Vignesh, M. Vijay

ABSTRACT

Comparison of analytical methods by Infrared Spectroscopy (IR) and High- Performance Liquid Chromatography (HPLC)..These comparison new simple, effective, easily understanding, rapid and specific analytical methods for the estimation of azithromycin IP drug.(Table.2) shows the results for azithromycin tablets achieved through the proposed method.The quantity of azithromycin active ingredient computed from analysed tablet sample was in the range of (90 - 105%) and matched with labelled data provided by manufactures. A new, fast, simple, and specific HPLC method using UV detection at 212 nm for determining percent content in Azithromycin powdered was developed and validated according to ICH guidelines. In addition, the lesser concentration of acetonitrile allowed a cost-effective analytical process.(Table.4) shows the results for azithromycin active ingredient computed from analysed tablet was in the range of (99.8 – 100.2) and matched with labelled data with provided by manufacture.We have reviewed how two common azithromycin potency estimation methods IR spectroscopy and HPLC chromatography. These two methods were evaluated using criteria of accuracy, speed, & cost. HPLC chromatography is accurate but can be slow and expensive to use, IR spectroscopy is typically not as accurate as HPLC chromatography, but is quicker with a lower cost as per analysis.

KEY WORDS:Comparison, HPLC, IR, Azithromycin.