Advances in Electrophoresis: A Review

Advances in Electrophoresis: A Review

Author Name : Dr. Manju Singh, Dr. Ramesh Dabas

Electrophoresis involves the migration of charged particle or molecules under the influence of an applied electric field. Biomolecules like peptides, amino acids, proteins, nucleic acid, and nucleotides contain ionizable groups, in solution as electrically charged particles may be as cations or anions a particular pH. Migration of ions depends on depends on molecular mass of the taken biomolecule, net charge density of the bio mole­cule taken and  shape of the taken biomolecule. Capillary electrophoresis has become prominent nowadays, because of its advantages over conventional electrophoresis techniques. It has fast analysis times, extremely high automation, online detection of separated peaks; thus, detection is a process that goes side by side with separation and not post-separation as usually in all the conventional techniques.