Development and Validation of HPLC Method for...

Development and Validation of HPLC Method for the Estimation of Flurbiprofen in Matrix Formulation

Author Name : Ms. Aachal S.Chimkar, Ms. Pratiksha R. Meshram, Mr. Ajinkya V. Gulhane, Ms. Sayali V. Pawar, Dr. Anil P. Dewani, Dr. Anil V. Chandewar, Ms. Radha S. Prayag

DOI: https://doi.org/10.56025/IJARESM.2023.1112232165

ABSTRACT A simple, sensitive, rapid and precise method for the estimation of Flurbiprofen in matrix tablet dosage form by using HPLC was developed and validated. The elution was achieved by using a Kintex EVO C18 (250 cm x 4.6 mm, 5micron) maintained at temperature of 28˚C, in isocratic mode of analysis, column was equilibrated by mobile phase Acetonitrile: Methanol : Potassium dihydrogen phosphate and Sodium dihydrogen phosphate (0.1%) in ratio of (45:5:50) The flow rate was set to 1.0mL/min and PDA detection done at wavelength of 247 nm. The retention time of Flurbiprofen was found to be 8.7 min. The method was validated for system suitability, specificity, accuracy, liniearity, robustness. The system suitability, specificity results were found within acceptance criteria for developed method. The method was linear in the range of 50 % to 150 %, with correlation coefficient 0.996. The percentage recovery for Flurbiprofen was found to be 98% and 99%.