Development and Validation of Lc-Ms/Ms Method...

Development and Validation of Lc-Ms/Ms Method for the Estimation of Acyclovir in Pharmaceutical Dosage Form

Author Name : Mr. Sandip V. Jadhav, Mr. Bhimashankar K. Bhui, Mr. Revanayya C. Swami, Mr. Ritesh K. Bansode, Ms. Rutuja A. Khot

ABSTRACT Using acyclovir as an internal standard, the study sought to validate a solid phase high-performance liquid chromatography method in conjunction with mass spectrometry for the measurement of acyclovir in human plasma. Using the column, the technique showed accuracy and precision within FDA standards. Acyclovir was consistently recovered on average from plasma samples. To treat and prevent initial and recurring episodes, acyclovir is taken orally. It is also known as 2-amino-1,9-dihydro-9- [(2hydroxyethoxy) methyl]-6Hpurine-6-one, or 9- [(2-hydroxyethoxy) methyl] guanine. Its molecular weight is 225.21 g/mol and its formula is C8H11N5O3. acyclovir as Internal Standard has a molecular weight of 255.23 g/mol and the chemical formula is C9H13N5O4. Double distillation and purification using the water purification equipment produced HPLC grade water. The buffer solution was prepared by diluting ammonium acetate to 1000 ml of Milli Q-water, adding formic acid to adjust the pH to 5.0, and filtering the mixture through a filter. The diluent solution of methanol and water (50:50%, v/v)], the mobile phase of ammonium acetate and acetonitrile (25:75%, v/v at pH 5.0), and the elution solution of acetonitrile and water (80:20%, v/v) were produced, filtered through Nylon (0.22µm) membrane sample filter paper, and then degassed. Every solution was kept in a room temperature storage.