Development and Validation of Stability Indic...

Development and Validation of Stability Indicating of RP-HPLC Method for Estimation of Topiroxostat in its Tablet Dosage Form

Author Name : Gajera Dipali Kishorbhai, Dr. Sibaji Sarkar, Mr. Dhirendra Tarai

ABSTRACT

An optimized isocratic method using RP- HPLC was performed and validated for Topiroxostat tablet dosage form. The new developed RP-HPLC method was highly precise, specific, reliable and accurate for the analysis of Topiroxostat tablet dosage form. Also the reduced retention time of drug peak shows the method was time saving and inexpensive. Mobile phase:  Mixed phosphate buffer: Acetonitrile (45:55). at 276 nm. Higher percentage recovery shows that the methods are free from interference of the excipients used in the commercial formulation. Results also showed that the developed methods can be applied to a routine analysis, quality control of mixtures and commercial preparations containing these drugs. Linearity range of 20-100 µg/ml and Accuracy (% Recovery) of 99.93 ± 0.925 to 99.947 ± 0.550 % and Limit of Detection (LOD) and Limit of Quantification (LOQ) of 3.101 µg/ml and 9.640 µg/ml of Topiroxostat

Keyword: RP-HPLC, Topiroxostat, FTIR. UV