Estimation of Cell-free Circulatory Neutrophi...

Estimation of Cell-free Circulatory Neutrophil Extracellular Traps (NETs) activation and Hypoxic inducible factor (HIF1α) in Ischemic brain patients

Author Name : Nusrath Fathima, Mohammed Zoheb, Naseem Begum, Aleem Ahmed Khan

DOI: https://doi.org/10.56025/IJARESM.2024.1208242486

ABSTRACT The immune system contributes to brain damage induced by ischemia, and the wounded brain has an immunosuppressive effect that promotes lethal infections, endangering people's lives after a stroke. Impaired cerebral perfusion causes acute hypoxia and glucose deprivation, which sets off a stroke cascade that eventually results in cell death. Neutrophil Extracellular Traps (NETs) play a major role in the formation of thrombi in the microenvironment which ultimately lead to the hypoxic accumulation. Hence, to evaluate the factors contributing to the decision of the neutrophil to form NETs we have assessed the cell free circulatory PADI4 and HIF-1α genes in the which is the key factor in cell response to hypoxia.A total of 32 Ischemic stroke patients and 32 healthy subjects have been included in the study, the blood plasma was collected from the patients who admitted within window period (4.5hours) of stroke. The total RNA as isolated using lab developed in-house method. cDNA preparation was done using OligodT and expression of PADI4 and HIF1 genes was using SYBR green master mix assay.The expression of PADI4 (p=0.03) and HIF1 (p=0.003) are increased in ischemic stroke patients as compared with control subjects. Further when compared PADI4 with HIF1 the PADI4 expression was high in ischemic stroke patients.Our findings imply that hypoxia-related genes and NETs are critical to the assortment and intricacy of the IS immunological microenvironment. Investigating the relationship between hypoxia-related essential genes and immune cells (NETS-PADI4) provides novel insights into ischemic stroke treatment targets.