In Vitro Anthelmintic Activity of Cleome Visc...

In Vitro Anthelmintic Activity of Cleome Viscosa Linn. Stembark on Indian Adult Earthworm

Author Name : Dr. Umesh Prabhakar Joshi, Mr. Chetan Vijay Jain, Dr. Jayashri A. Patil, Dr. Kailaspati Prabhakar Chittam

DOI: https://doi.org/10.56025/IJARESM.2023.1162326

ABSTRACT

Objective: Gastrointestinal nematodes are the major restrictive factor for the successfulness of livestock production all over the world. Appearance of resistance strains as well as scarcity and high cost of the currently available drugs has lead to the evaluation of other alternative helminth control options, mainly from plants. The development of anthelmintic activity and the high cost of synthetic anthelmintic drugs it leads to the evaluation of medicinal plants as an alternative source of anthelmintics. In the current study, in vitro experiments were conducted to determine the possible anthelmintic effects of crude Chloroform, Methanolic, Aqueous and Hydroalcoholic extracts of the Leaves of the plant Cleome viscosa Linn. on eggs and adult Pheretima posthuma and Ascaridia galli. Methods: Three concentrations (10, 25 and 50 mg/ml) of chloroform, methanolic, aqueous and hydroalcoholic extracts of stembark of plant Cleome viscosa Linn. were studied in activity which involved the determination of the time of paralysis (called as vermifuge) and time of death (called as vermicidal) of the worms. Piperazine citrate in same concentration as those of extract was included as standard reference and normal saline water with 1% carboxymethyl cellulose as control.