Isolation and Identification of Actinomycetes...

Isolation and Identification of Actinomycetes Producing β-Mannanase

Author Name : Zine A.S, Peshwe S.A

ABSTRACT

Isolation of potential actinomycete capable of degrading mannan was explored from various sources of soil sample collected from agricultural areas. The isolates were screened based on the clearing zone method on selective agar media containing guar gum as substrate. A total of 30 isolates were screened and only 07 had activity ratio ranging from 1.5 mm to 3.5 mm which were used for further analysis. These isolates were incubated in shake flasks at 45 °C for 96 h having minimal media and 1% guar gum as substrate. The best enzyme producer was isolate AZA12 with mannanase enzyme acitivity of  165.5 U/ml. The isolate AZA12 was identified as Streptomyces flaveoulus and was further used for the production of mannanase from agroresidues. Different agroresidues were screened for mannanase production of which soy straw supported maximum mannanase production with enzyme activity of   80.18 U/ml.

Key words: agroresidue, guargum, mannan.