Isolation and Indentification of Glycerides o...

Isolation and Indentification of Glycerides of Bauhinia Variegata Plant Found in Manipur Followed by Transetrification and Characterization of the Same Glycerides into Biodiesel

Author Name : Md. Abdul Halim Shah, Dr. Naorem Sarita Devi

ABSTRACT Seedoil of Bauhinia variegata was extracted by Solvent extraction technique on the crushed kernel using petroleum ether as the solvent. The oil was purified prior to further analysis by column chromatography over silica gel (60-120 mesh) using a mixture of petroleum ether and ethyl acetate (20:1) as the eluent. The purified oil was further transesterified giving the final product known as biodiesel. The biodiesel is also known as the fatty acid methyl ester (FAME).Fatty acid methyl ester (FAME) composition of Bauhinia variegata determined by NMR, IR and GC-MS analysis. The FAME of Bauhinia variegata consists of 19.82 wt% of methyl palmitate (C16:O), 60.78 wt % of methyl oleate (C18:1), 17.53 wt% of methyl stearate (C18:O) and 1.87 wt% of methyl arachidate ( C20:0)