Method Development and Validation of Acyclovi...

Method Development and Validation of Acyclovir By RP-HPLC

Author Name : Dr. R Radha, Hima Bindu Nettem

ABSTRACT

The present work is aimed to develop a simple, rapid-fire, reproducible, dependable and effective retrograde phase high performance liquid chromatographic (RP-HPLC) system for estimation of a antiviral medicine Acyclovir in raw material and its tablet lozenge form. Separation was done by using mobile phase conforming of HPLC grade water and methanol in the rate of 5050. The separations were carried out on Welchrom C18 column (250 x4.6 mm; 5 µm) Shimadzu LC-20AT Prominence Liquid Chromatograph. The inflow rate was set at 1 mL/ min. The injection volume was 20 µL and the UV sensor was operated at 250 nm using Shimadzu SPD-20A Prominence UV-Visible sensor. The retention time of Acyclovir was plant to be3.077 twinkles. The standard estimation plot was plant direct over the range of 2 to 10 µg/ mL and the measure of correlation was plant to be (r2 = 0.9999). The RSD values of intraday and interday perfection were plant below two which indicates that the system was largely precise. The LOD and LOQ were plant to be0.247 and0.74 independently. The advanced system was ultimately applied for quantification of retailed expression. Satisfactory results were attained. The mean assay values of Acyclovir in good agreement with the marker claim. The advanced system was validated according to transnational conference on adjustment (ICH) guidelines for particularity, linearity, range, delicacy, perfection, discovery limit, quantitation limit, robustness and system felicity parameters and the results attained were satisfactory. The system has been successfully used to dissect solid lozenge containing Acyclovir with good reclamations and proved to be robust.

KEYWORDS: RP - HPLC, Acyclovir, Validation, ICH guidelines.