“Method Development and Validation of Fosin...

“Method Development and Validation of Fosinopril Sodium Anti Hypertensive Drug By RP-HPLC”

Author Name : Snehal More, Dr. Narendra M. Gowekar

ABSTRACT

Using high-performance liquid chromatography, a robust and rapid analysis of Fosinopril Sodium system was designed and validated.  The assay was performed using the C18 Agilent- Analytical Column (5 μm, 250 × 4.6 mm) Methanol and water (0.1%withOPA pH 3.3) 70 : 30 were used in the mobile phase, which had a flow rate of 0.7 mL/min and a detection wavelength of 267 nm (adjust pH 3 (50:50 V/V).  The injection volume was 20 µL. The retention times for Fosinopril Sodium were 4.543 minutes. Fosinopril Sodium concentrations (10-50 g/ml) were tested throughout a wide range of concentrations. The Fosinopril Sodium all validation criteria and system appropriateness tests were carried out rigorously. (R2 = 0.999) The analytical graph was linear. With a relative standard deviation of less than 2.0 percent, the system demonstrates sufficient accuracy. With a percent RSD of less than 2%, the approach demonstrated good duplicability and recovery. As a result, the suggested system was discovered to be simple, precise, accurate, linear, and tough. As a result, it may be used to analyse Fosinopril Sodium in bulk drugs in practice.

 KEY WORDS: Fosinopril Sodium,Validation, RP-HPLC.