Method Development and Validation of Pesticid...

Method Development and Validation of Pesticides Residue in Rice by LC-MS/MS

Author Name : Shaily Raizada, Dr. Rahul Kumar

ABSTRACT

A LC-MS/MS method was developed for determination of residual pesticides using Zorbax Eclipse Plus C18 (150X4.6mm), 3.5μm and a mobile phase of 5mM Ammonium Formate in Solution A and 5mM Ammonium Formate in Solution B with gradient, at flow rate of 0.6 ml/min with MS detector. The column oven temperature was 450C and the polarity was ESI positive and ESI negative. The proposed method was validated for System suitability, Specificity, Linearity, LOD and LOQ determination, Recovery, Precision, Range and Robustness. All the parameters were found within the acceptable limits. Linearity of residual pesticides was in the range of 2.5-100μg/kg. LC-MS/MS method was specific, accurate, precise and suitable for the analysis of residual pesticides in Rice.

Keywords: Liquid chromatography with mass spectrometry (LC-MS/MS), Residual Pesticides, Sanco guideline and Method Validation.