Supercritical Fluid Chromatography

Supercritical Fluid Chromatography

Author Name : Bestha Lakshmi Kalyani, Mamidi Adarsh, Vadladi Nikhila, Jestadi` Raga Swetha, Gandrathi Srujana

ABSTRACT SFC is a combination of higher performance liquid chromatography [HPLC] and Gas chromatography [GC].A supercritical fluid chromatography is a material that can be either liquid or gas used in state above critical temperature or critical pressure where gases and liquid can coexist, is known as supercritical fluid chromatography. SFC has been used primarily for separation of chiral molecules, mainly those which require Normal Phase conditions. While the mobile phase is a fluid in the supercritical state, the stationary phase is packed inside columns similar to those used in liquid chromatography.Supercritical fluid extraction (SFE) is a method of extracting a sample utilising the unique properties of supercritical fluids. A supercritical fluid diffuses quickly as a gas and is capable of dissolving materials as a liquid. Supercritical carbon dioxide is mainly used.SCF technology is based on the principle of precipitation of drug or microparticles using a compressed antisolvent such as supercritical carbon dioxide. The solutes are dissolved in a solvent. Keywords: SFC [Supercritical fluid chromatography], SFE [Supercritical fluid extraction].