Synthesis, Characterization and Biological Ac...

Synthesis, Characterization and Biological Activity of 1, 3, 4- Oxadiazole Analogues as Anti-Microbial Activity

Author Name : Dipali Deshmukh, Sarita Singh Ahirwar, Dr. Navjot Singh, Dr. Anwar Iqbal Khan

ABSTRACT The objective of present research work is synthesis, characterization and biological activity of 1,3,4- oxadiazole analogues as anti-microbial activity. Synthesis of Oxadiazole & it 5 derivatives was done and structure of synthesized Oxadiazole Derivatives & it 5 derivatives was confirmed using spectral as well as physical method data. Disappearance of absorption bands in synthetic derivatives at 3414(-NH2 N-H str.), 3260 (-NH- N-H str.) which is present in ODA-0, and appearance of bands between 1640-1690 cm-1 of imine -C=N-(Schiff base) in FT-IR spectrum clearly indicated. This fact was further supported by 1HNMR spectrum, disappearance of NH2 peak from different synthesized derivatives, peak at 8.35 or 8.59 of imine -C=N-(Schiff base), 7.53 and 7.41 ppm of aromatic hydrogen were consistently appeared in all 5 synthesized derivatives. Moreover, synthesized compounds characterized by the TLC and melting point. In addition, by visual inspection synthesized compounds also characterized. Synthesized Oxadiazole derivatives (All five) were screened for in vitro antimicrobial activity against gram-positive bacteria B. Subtilis and gram-negative bacteria and E. Coli, cutaneous fungi C. Albicans and A. Niger. Synthesized Oxadiazole ODA-1 exhibited excellent antibacterial activity, ODA-2 and ODA-3 exhibited moderate antibacterial activity against B. subtilis in comparison to standard drug – Ciprofloxacin. ODA-4 and ODA-5 exhibited Poor antibacterial activity against B. subtilis. Synthesized Oxadiazole ODA-1, ODA-2 and ODA-3 were exhibited moderate antibacterial activity against E. coli in comparison to standard drug – Ciprofloxacin. ODA-4 and ODA-5 exhibited Poor antibacterial activity against E. coli. Analysis of the antifungal activity data concluded that ODA-3 exhibited excellent antifungal activity, ODA-1, ODA2 exhibited moderate antifungal activity and ODA-4 and ODA-5 possessed significant antifungal activity against fungus Candida Albicans when compared to the reference drug griseofulvin.