UV-Spectrophotometric and Stability Indicatin...

UV-Spectrophotometric and Stability Indicating Rp Hplc Method Development and Validation for Simultaneous Estimation of Pyrentel Pamoate and Praziquantel in the Pharmaceutical Dosage Form.

Author Name : Jamtani Pooja, Mr. Dhaval Patel, Dr. Hiral Panchal

ABSTRACT

A reverse phase high performance liquid chromatographic method was developed for the validation of Pyrantel Pamoate and Praziquantel in Pharmaceutical Dosage Form. The separation was achieved by C18 (250 X 4.6 mm, 5 μm) column and buffer: acn(60:40) as mobile phase, buffer{0.05m KH₂po 4 IN H2O  ph 3 (tea)} at a flow rate of 1 ml/min. Detection was carried out at 220 nm. Retention time of Pyrantel Pamoate and Praziquantel were found to be 2.271 and 4.943 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity for Pyrantel Pamoate is in range of 50-150 μg/ml and Praziquantel is in the range of 25-75 μg/ml. The accuracy of the methods was assessed by recovery studies and was found to be 98-102 %. Developed method was found to be accurate, precise, linear and robust for Pyrantel Pamoate and Praziquantel in Pharmaceutical Dosage Form. The drug was subjected to stress condition of hydrolysis, oxidation, photolysis and Thermal degradation, Considerable Degradation was found in alkaline degradation. The proposed method was successfully applied for the estimation of Pyrantel Pamoate and Praziquantel in Pharmaceutical dosage form.

Keywords: Pyrantel Pamoate, Praziquantel, Stability Indicating RP-HPLC Method, Validation, Pharmaceutical dosage form.