UV Spectrophotometric Method Development and ...

UV Spectrophotometric Method Development and Validation of Bilastine in Bulk and Tablet Formulations

Author Name : Ankita Shinde, Dr. G.B. Gajeli, Yogesh Hanchate, Anita Shegaonkar

ABSTRACT

Objective: A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for Bilastinein Tablet Formulation.

Methods: The UV spectrum of Bilasine in Methanol 0.1%: TFAwater(80:20) showed λ max at 277 nm. Beer’s law is valid in the concentration range of 8-40 µg/ml. This method was validated for linearity, accuracy, precision, LOD and LOQ.

Results: The method has demonstrated excellent linearity over the range of 8-40µg/ml. with regression equation y = 0.0198x + 0.0814 and regression correlation coefficient r²= 0.999. Moreover, the method was found to be highly sensitive with LOD (1.57μg/ml) and LOQ (4.77μg/ml).

Conclusion: Depending on results the given method can be successfully applied for assay of Bilastine in Tablet formulation.

Keyword: Bilastine, UV spectroscopy, method development and validation, Methanol TFA: Water, Tablet Formulation.