UV Visible Spectroscopy Method Development an...

UV Visible Spectroscopy Method Development and Validation

Author Name : Nehal Upadhyay, Prof. Mitali Dalwadi, Dr. Umesh Upadhyay

ABSTRACT UV-Visible spectroscopy is a widely used analytical technique in various fields such as pharmaceuticals, environmental science, food industry, and materials science. This review explores the methodologies employed in the development and validation of UV-Visible spectroscopic methods. The focus is on discussing the key steps involved in method development, including selection of appropriate solvent, determination of suitable wavelength, optimization of instrumental parameters, and validation of the developed method according to regulatory guidelines. A significant portion of the review is dedicated to method validation, focusing on parameters such as accuracy, precision, linearity, robustness, etc. In addition to method development and validation, the review explores various estimation techniques utilizing UV-Visible spectroscopy. These include Simultaneous Estimation, Q Absorbance Ratio, Derivative Spectroscopy, and Difference Spectroscopy. Overall, this review serves as a comprehensive resource for researchers and practitioners involved in UV-Visible spectroscopy, providing valuable insights into method development, validation, and estimation techniques